Journal: Iranian Journal of Basic Medical Sciences

Article Title: Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation

doi: 10.22038/ijbms.2021.57196.12736

Figure Lengend Snippet: Representative images of the effect of Ura treatment after ovarian T/D injury (x20, IHC): Arrows show IL-1β immune positive cells. a) sham group with negative IL-1β immunopositivity of the lutein and interstitial cells. b) T/D group with intense IL-1β immunopositivity of lutein and interstitial cells. c) T/D+DMSO group with more intense IL-1β immunopositivity of lutein and interstitial cells. d) T/D+Ura 0.5 mg/kg group with moderate IL-1β immunopositivity of lutein and interstitial cells. e) T/D+Ura 5 mg/kg group with mild IL-1β immunopositivity of lutein and interstitial cells

Article Snippet: Cleaved caspase-3 (Novus Biological, Cat. No: NB600-1235, Dilution: 1/100), light chain 3B (LC3B) (Abcam, Cat. No: ab48394 Dilution: 1/200), nuclear factor kappa-B (NF-κB) (Abcam, Cat No: ab7971, Dilution: 1/150), tumor necrosis factor-alpha (TNF-α) (Novus Biological, Cat. No: NBP1-19532, Dilution: 1/100) and interleukin-1beta (IL-1β) (Bioss, Cat. No: bs-0812R, Dilution: 1/100) were applied as the primary antibody.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation

doi: 10.22038/ijbms.2021.57196.12736

Figure Lengend Snippet: Representative images of the effect of Ura treatment after ovarian T/D injury (x20, IHC): Arrows show TNF-α immune positive cells. a) sham group with negative TNF-α immunopositivity of lutein and interstitial cells. b) T/D group with more intense TNF-α immunopositivity of lutein and interstitial cells. c) T/D+DMSO group with intense TNF-α immunopositivity of the interstitial cells. d) T/D +Ura 0.5 mg/kg group with mild TNF-α immunopositivity of the lutein cells. (e) T/D +Ura 5 mg/kg group with mild IL-1β immunopositivity of the interstitial cells

Article Snippet: Cleaved caspase-3 (Novus Biological, Cat. No: NB600-1235, Dilution: 1/100), light chain 3B (LC3B) (Abcam, Cat. No: ab48394 Dilution: 1/200), nuclear factor kappa-B (NF-κB) (Abcam, Cat No: ab7971, Dilution: 1/150), tumor necrosis factor-alpha (TNF-α) (Novus Biological, Cat. No: NBP1-19532, Dilution: 1/100) and interleukin-1beta (IL-1β) (Bioss, Cat. No: bs-0812R, Dilution: 1/100) were applied as the primary antibody.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation

doi: 10.22038/ijbms.2021.57196.12736

Figure Lengend Snippet: Representative images of the effect of UR treatment after ovarian torsion detorsion injury (x20, IHC): Arrows show LC3B immune positive cells. (a) sham group with negative LC3B immunopositivity of lutein and interstitial cells. (b) T/D group with intense LC3B immunopositivity of lutein and interstitial cells. (c) T/D+DMSO group with intense LC3B immunopositivity of the lutein cells. (d) T/D +Ura 0.5 mg/kg group with intense LC3B immunopositivity of lutein and interstitial cells. (e) T/D +Ura 5 mg/kg group with mild IL-1β immunopositivity of the interstitial cells

Article Snippet: Cleaved caspase-3 (Novus Biological, Cat. No: NB600-1235, Dilution: 1/100), light chain 3B (LC3B) (Abcam, Cat. No: ab48394 Dilution: 1/200), nuclear factor kappa-B (NF-κB) (Abcam, Cat No: ab7971, Dilution: 1/150), tumor necrosis factor-alpha (TNF-α) (Novus Biological, Cat. No: NBP1-19532, Dilution: 1/100) and interleukin-1beta (IL-1β) (Bioss, Cat. No: bs-0812R, Dilution: 1/100) were applied as the primary antibody.

Techniques:

Journal: Diabetology & Metabolic Syndrome

Article Title: Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance

doi: 10.1186/s13098-023-01244-1

Figure Lengend Snippet: Antibodies

Article Snippet: IL-6 , Rabbit , Bioss , bs-4539R.

Techniques:

Journal: Annals of Translational Medicine

Article Title: LncRNA NEAT1 mediates intestinal inflammation by regulating TNFRSF1B

doi: 10.21037/atm-21-34

Figure Lengend Snippet: NEAT1 promotes the translocation of NF-κB p65 by regulating TNFRSF1B and participates in TNF-α mediated inflammation. (A) Translocation of NF-κB p65 to the nucleus in NCM460, NF-κB p65 (green) and DAPI stained nuclei (blue) under 200× field. (B) Translocation of NF-κB p65 to the nucleus in HT-29 under 200× field. (C) The protein expression levels of TNFRSF1B, NF-κB p65 and p-NF-κB p65, as well as proliferation and inflammation-related factors IL1R1 and Caspase-4 in different groups. NEAT1, nuclear enriched abundant transcript 1; IL1R1, interleukin 1 receptor type I; TNFRSF1B, tumor necrosis factor superfamily member 1B; TNF-α, tumor necrosis factor-alpha; NF-κB, nuclear factor-kappa B.

Article Snippet: The detailed list of antibodies are as follows: TNFRSF1B Rabbit pAb (1:1,000; cat. no. 19272-1-AP; Proteintech Group, Inc.), NF-κB p65 Rabbit pAb (1:1,000; Cat. No. 10745-1-AP; Proteintech Group, Inc.), p-NF-κB p65 Rabbit pAb (1:1,000; cat. no. ab28856; Abcam, Inc.), β-actin Mouse mAb (1:2,000; cat. no. 66009-1-Ig; Proteintech Group, Inc.), IL1R1 Rabbit pAb (1:1,000; cat. no. bs-20697R; Bioss, Inc.), Caspase-4 Rabbit pAb (1:2,000; cat. no. BA2212; Boster, Inc.) followed with corresponding HRP-conjugated Affinipure Goat-Rabbit/Mouse IgG Secondary Antibody (1:4,000; cat. no. SA00001-2/SA00001-2; ProteinTech Group, Inc.) incubated at 37 °C for 1 h, and finally luminescence solution was added.

Techniques: Translocation Assay, Staining, Expressing